Modul 3E - Production of transgenic animals: biological fundamentals, planning and practical considerations

This two days course provides the participants with theoretical knowledge and tools for generation of gene-modified animals

 

Participants of the course will learn:

  • The different techniques for generation of gene-modified animals in small rodents and large animals: Conventional Transgenes, ES cell-Technology, Cre-loxP system, gene editing, and cloning
  • Practical considerations for planning transgenes and gene targetings
  • Practical considerations for conducting CRISPR/Cas9-mediated gene editing
  • All planning steps required for generating a new gene-modified animal
  • Reproductive aspects of generation of gene-modified animals
  • Ethical and 3R aspects of generation and maintenance of gene-modified animals
  • Nomenclature and reproducibility in the context of work with gene-modified animals
  • Legal requirements of generation and maintenance of gene-modified animals

 

After lectures introducing the basics in these topics participants solve practical problems typically arising during the course of a project involving generation of new gene-modified animal (internally or externally). The solutions will be discussed.

Participants of the course will be able to plan a new gene-modification with a specific scientific purpose and supervise/control the work of a transgenic facility or external service provider.

This course is addressing PhD students, post-docs and senior researchers. 

 

Other relevant courses: Module 5E and Module 15E

 

For more details, please read the full description of this course in this link.

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LINK TO DETAILLED INFORMATION - DATES - REGISTRATION