Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations
An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms.
Gantz VM, Bier E. Science. 2015 Apr 24;348(6233):442-4.
More news
- CRISPR off-target analysis in genetically engineered rats and mice
- The most popular genes in the human genome
- Correction of a pathogenic gene mutation in human embryos
- Easi-CRISPR
- Ethical issues of CRISPR technology and gene editing through the lens of solidarity
- Reconstitution in vitro of the entire cycle of the mouse female germ line
- Healthy ageing of cloned sheep
- Photo-activatable Cre recombinase regulates gene expression in vivo
- Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
- High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
- In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy